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Description

After TMB substrate solution is added, only those wells that contain Human SPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

doi: 10.1016/j.bmcl.2004.09.067 57

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Brain J Neurol 135(Pt 9):27982808

Maes M, Mihaylova I, Kubera M, Leunis JC, Twisk FN, Geffard M

Aliquots (0.5 mL) of the supernatant were mixed with 0.5 mL of 10 mM phosphate buffer (pH 7.0) and 1.0 mL of 1 M potassium iodide, and absorbance was measured at 390 nm